The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. dextran Small Intestinal Permeability and Gut-Transit Time Determined The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. There is a deviation from linearity after 580kDa [69, 70]. 4K, b M.W. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. In addition, we evaluated the regeneration of SC after microporation. He, L.; Niemeyer, B. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Chem. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Stokes radius Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. (2012). Journal of Applied Polymer Science, 72, 871-876. MeSH terms The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). 2015;32(5):170413. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. ; Bataille, B. The micropore formed at the SC through to the epidermis, enabling drug delivery. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. This study was supported by the LG Electronics. Advanced Drug Delivery Reviews, 60, 1650-1662. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. "Dextrans in Aqueous Solution. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. (2013). Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. 2012;101(8):28619. Monolayers and Thin Films of Dextran Hydrophobically Modified. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. 2009;8(2):1739. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Herwadkar A, Banga AK. Eng. Ibrahim O, Munavalli GS, Dover JS. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. WebDextran 40 Pharmaceutical Quality. Microporation techniques for enhanced delivery of therapeutic agents. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Intrinsic viscosity of bovine serum albumin conformers. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Carbohydrate Polymers, 68, 477-488. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Citation-(RIS (2001). De Belder, A. N. (2001). The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Thermo Fisher Scientific. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Food Funct. Determination of polymer structure by Gel Permeation Chromatography. MeSH 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Hydrodynamic Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Regulating dynamic signaling between hematopoietic stem cells A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Data File Dextran. FITC-dextran solution (600L) was added to the donor chamber. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. (2003). However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). J Cosmet Laser Ther. By using this website, you agree to our The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Formation of dextran hydrogels by crystallization. European Polymer Journal, 43, 1744-1753. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. International Journal of Biological Macromolecules, 42, 133-137. Nat Biotechnol. Granath, K. A. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Cell permeabilization using a non-thermal plasma - IOPscience ; Ross-Murphy, S.B. ; Picout, D.R. Table 2 compares the properties of nanoparticles coated with one or other polymer. 2013;43(4):287303. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free In fact, short chains do contain minor branching points. Epub 2017 Jun 1. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. European. Clipboard, Search History, and several other advanced features are temporarily unavailable. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Learn More. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Curvale, R.; Masuelli, M.; Perez Padilla, A. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Gold MH, Biron JA. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. eCollection 2015. Nanoparticles in magnetic resonance imaging: from simple to dual Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. The authors declare that they have no competing interests. ; Ghosh, T.K. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Molecular weight, molecular weight distribution and molecular size of a native dextran. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). dextran hydrodynamic radius ; Talsma, H.; Hennink, W.E. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Hydrodynamic Drug Deliv. The above data are not useful because it can not be compared with those obtained in this work. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. The basic setup of a DLS instrument is shown in Figure 1. ; Prasad, D.H.L. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. dextran hydrodynamic radius (2004). Fluorescent and Biotinylated DextransSection 14.5 Exp Cell Res. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Dilute solution properties and degree of chain branching for dextran. PubMedGoogle Scholar. The Mark-Houwink. 2000;3(9):31826. Ahn, G.Y., Eo, HS., Kim, D. et al. Carbohydr Polym. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Carbohydrate Polymers, 36, 319-326. TEWL measurements were taken 30min after microporation of the human and pig skin. Sung-Wook Choi. (1987). Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Carbohydrate Research, 340, 811-826. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Article The Transdermal diffusion cell system was protected from light. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. The genus Weissella: taxonomy, ecology and biotechnological potential. Dilute solution properties and degree of chain branching Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. (1999). Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Please enable it to take advantage of the complete set of features! For data requests, please contact the authors. and transmitted securely. Terms and Conditions, In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. (2003). However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Figure4 shows the histological analysis of micropores in human cadaver skin. Radius Beer, M.U. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. This site needs JavaScript to work properly. 2016;9706:97061E6. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Kasaai, Mohammad R. (2012). Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Structure Properties of Dextran. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity.